MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

液相色谱法测定牛奶中三聚氰胺残留量的不确定度

通过数学建模,对GB/T22388-2008中HPLC法测定牛奶中三聚氰胺残留量的不确定度进行分析。将各个分量分化,运用最小二乘法对标准曲线拟合的不确定度,用极差法对测定次数较少时引起的不确定度,添加回收率的不确定度等进行分析。结果表明,合成标准不确定度U（X）=0.040,当取样量为2.00g,k=2（95%置信度）时,测得牛奶中三聚氰胺的含量为（2.00±0.160）mg/kg。     

水培条件下钾对大蒜幼苗生长及根系活力的影响

以‘金乡白皮蒜’为试材,采用设施水培探讨了不同K+浓度对大蒜幼苗生长及根系活力的影响。结果表明:钾可显著增加大蒜幼苗生长量及根系活力;当营养液K+浓度为6.0mmol/L时,大蒜幼苗叶长、叶宽、假茎高、假茎粗、根长及根系活力最大,绿叶数及根系数最多,大蒜幼苗叶片、假茎、鳞茎及根系干、鲜重也最大,较不施钾处理干重分别增加133.91%、167.22%、146.31%和116.74%,鲜重分别提高168.34%2、43.18%、153.24%和128.78%。     

高辣椒素含量航天辣椒桂航一号的选育

桂航一号是红椒一号经过太空诱变处理后,单株选育3代、混合选育2代育成的高辣椒素含量辣椒新品种。该品种中晚熟,从定植至采收红椒约90d。株高90cm左右,植株开展度85cmx85cm左右。第一花着生13—14节。果实羊角形,纵径15～16cm,横径1．3cm,肉厚0．19cm,2—3心室。果肩平或斜,果顶锐尖,果面光滑,果皮薄,果形较直,青果深绿色,生物学成熟果鲜红色,平均单果质量12～13g,最大单果质量15g。果实每100g鲜质量维生素C含量87．9mg,辣椒素含量104mg／kg,干物质含量8．56％。其中辣椒素含量比红椒一号（5．55mg／kg）提高了近20倍,解决了红椒一号作为辣椒酱原料辣度不够的缺点。667m2产量3942．1奴,较红椒一号增产15．8％,达极显著水平。植株外被茸毛,较耐炭疽病、疫病、病毒病,抗高温,耐湿能力强,适于嗜辣地区作鲜食、加工盐渍、酱制或干制栽培。     

高海拔地区夏季双孢蘑菇栽培优良菌株的筛选

通过对引进的9个双孢蘑菇菌株和当地主栽品种As2796的菌丝生长特性、生育期、子实体形状、产量和生物学效率等的比较,结果表明,双孢蘑菇菌株双13、991和Ag23综合性状表现较好,出菇早,商品性好,生物学效率高,分别达到52.5%、51.8%和48.6%,较对照As2796增产10.0%、8.4%、1.7%。初步认为3个菌株适合在高海拔地区夏季生产中应用。     

钾肥水平对油茶果实性状及产量的影响

为了研究钾素水平对油茶果实性状和产量的影响及它们之间的关系,设置了6个不同钾肥用量处理,以8年生的长林53号和长林27号两个油茶无性系为试材在江西永丰开展了施肥试验,连续两年测定了油茶果径、单果质量、干出籽率、干出仁率、种仁含油率、鲜果含油率、单株结果数、单株果实产量和产油量等指标,比较和分析了不同无性系的不同处理之间的差异。结果表明,两个无性系的果实性状和产量对钾肥水平差异的响应均存在大小年差异;结实大年时,钾肥水平对两个无性系果实的果径、单果质量和干出仁率影响不大,对其他性状的影响均达到显著水平;结实小年时,钾肥水平仅对53号无性系单株结实数量和产量造成影响且无明显梯度规律,而27号无性系随着钾肥水平的提高,其干出籽率和鲜果含油率呈先上升后下降趋势,单株果实数量、产量和产油量则呈下降趋势;无论大年还是小年,单株产量均与结实数量关系最密切,大年的产量在油茶生产中占据主导地位,就大小年平均产量而言,两品系均以中等钾肥水平的K2和K3处理最优。适量增施钾肥可有效改善油茶结实大年果实性状,提高单株结实数量,实现增产增效。     

高温水供热干燥速生杉木方材

运用高温水供热系统干燥速生杉木方材。生产性试验表明，6天内初含水率为55．5％的杉木方材干至始含水率5．4％，干燥质量符合细木工板生产的要求（少数高含水率木材除外）。此干燥法集中供热，具有热效率高、运行安全和经济可行等特点。     

森林高火险预警的探讨

统计分析了句容市森林火灾情况，探讨了进行森林火险预报思路、因子初选及资料的分析处理，预报因子的筛选，建立线性方程组，求解预报方程并采用威尔克斯方法进行了判别函数总效果检验，最后提出了预报决策。     

以人为本，创造高等公路景观

文章从人的角度出发简要探讨了高等级公路景观的内涵，从景观设计的原则、内容方面就高等级公路的景观设计展开论述，然后从生态环境和生活环境出发对高等级公路的景观设计进行了剖析。